HemN

• Description: anaerobic coproporphyrinogen III oxidase
  
  

Gene name	hemN
Synonyms	yqeR
Essential	no
Product	anaerobic coproporphyrinogen III oxidase
Function	heme biosynthesis
MW, pI	41 kDa, 5.534
Gene length, protein length	1098 bp, 366 aa
Immediate neighbours	hrcA, lepA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis of cofactors

The gene

Basic information

• Locus tag: BSU25500

Expression

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Coproporphyrinogen-III + 2 S-adenosyl-L-methionine = protoporphyrinogen-IX + 2 CO₂ + 2 L-methionine + 2 5'-deoxyadenosine (according to Swiss-Prot)

• Protein family: anaerobic coproporphyrinogen III oxidase family (according to Swiss-Prot)

• Paralogous protein(s): HemZ

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P54304

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: lepA-hemN PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information: There is a terminator between lepA and hemN with only little readthrough

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

G Homuth, A Rompf, W Schumann, D Jahn
Transcriptional control of Bacillus subtilis hemN and hemZ.
J Bacteriol: 1999, 181(19);5922-9
[PubMed:10498703] [WorldCat.org] [DOI] (P p)

B Hippler, G Homuth, T Hoffmann, C Hungerer, W Schumann, D Jahn
Characterization of Bacillus subtilis hemN.
J Bacteriol: 1997, 179(22);7181-5
[PubMed:9371469] [WorldCat.org] [DOI] (P p)

G Homuth, M Heinemann, U Zuber, W Schumann
The genes of lepA and hemN form a bicistronic operon in Bacillus subtilis.
Microbiology (Reading): 1996, 142 ( Pt 7);1641-9
[PubMed:8757728] [WorldCat.org] [DOI] (P p)