QdoI

• Description: Fe-containing quercetin 2,3-dioxygenase
  
  

Gene name	qdoI
Synonyms	yxaG
Essential	no
Product	Fe-containing quercetin 2,3-dioxygenase
Function	resistance to plant product quercetin
MW, pI	37,4 kDa, 5.562
Gene length, protein length	1011 bp, 337 aa
Immediate neighbours	yxaH, qdoR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

resistance against other toxic compounds (nitric oxide, phenolic acids, flavonoids, oxalate)

This gene is a member of the following regulons

LmrA regulon, QdoR regulon

The gene

Basic information

• Locus tag: BSU39980

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: oxidation of the flavonol quercetin with dioxygen, cleaving the central heterocyclic ring and releasing CO

• Protein family:bicupin family

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): Mn(II) (preferred), but also active with Fe(II) snd Co(II) PubMed

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2H0V

• UniProt: P42106

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: qdoI-yxaH PubMed

• Sigma factor:

• Regulation:
  • induced by flavonoids such as quercetin (QdoR, LmrA) PubMed

• Regulatory mechanism:
  • LmrA: transcription repression PubMed
  • QdoR: transcription repression PubMed

• Additional information:
  • major regulator: QdoR PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

J B Broderick
Catechol dioxygenases.
Essays Biochem: 1999, 34;173-89
[PubMed:10730195] [WorldCat.org] [DOI] (P p)

T D Bugg, J Sanvoisin, E L Spence
Exploring the catalytic mechanism of the extradiol catechol dioxygenases.
Biochem Soc Trans: 1997, 25(1);81-5
[PubMed:9056848] [WorldCat.org] [DOI] (P p)