PutP

• Description: high affinity proline permease
  
  

Gene name	putP
Synonyms	ycgO
Essential	no
Product	high affinity proline permease
Function	proline uptake
MW, pI	48 kDa, 8.619
Gene length, protein length	1347 bp, 449 aa
Immediate neighbours	putC, putR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

transporters/ other, biosynthesis/ acquisition of amino acids, utilization of amino acids membrane proteins

This gene is a member of the following regulons

PutR regulon

The gene

Basic information

• Locus tag: BSU03220

Phenotypes of a mutant

• no growth with proline as single source of carbon or nitrogen PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): OpuE

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P94392

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:
  • putB-putC-putP PubMed
  • putP (internal promoter) PubMed

• Sigma factor:
  • putB: SigA PubMed

• Regulation:
  • induced in the presence of proline (PutR) PubMed

• Regulatory mechanism:
  • PutR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Boris R Belitsky
Indirect repression by Bacillus subtilis CodY via displacement of the activator of the proline utilization operon.
J Mol Biol: 2011, 413(2);321-36
[PubMed:21840319] [WorldCat.org] [DOI] (I p)