Pyk

• Description: catalyzes the transfere of a phosphate from phosphoennolpyruvate to ADT and synthesis of pyruvate
  
  

Gene name	pyk
Synonyms	pykA
Essential	no
Product	Pyruvate kinase
Function	kinase, transferase
MW, pI	61,9 kDa, 4.88
Gene length, protein length	1755 bp, 585 amino acids
Immediate neighbours	pfkA, ytzA
Gene sequence (+200bp)	Protein sequence
Genetic context

The gene

Basic information

• Coordinates:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + pyruvate = ADP + phosphoenolpyruvate

• Protein family: pyruvate kinase family, (C-terminal section: PEP-utilizing enzyme family)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation

• Cofactor(s): magnesium ion, potassium

• Effectors of protein activity:

• Interactions: Pyk-PfkA

• Localization: cytoplasm PubMed

Database entries

• Structure:

• Swiss prot entry: [3]

• KEGG entry: [4]

• E.C. number: [5]

Expression and regulation

• Operon: pfkA pyk

• Sigma factor:

• Regulation: twofold induced by glucose

• Regulatory mechanism:

• Additional information:

Labs working on this gene/protein

Jörg Stülke, University of Göttingen, Germany Homepage

References

1. Ludwig, H., Homuth, G., Schmalisch, M., Dyka, F. M., Hecker, M., and Stülke, J. (2001) Transcription of glycolytic genes and operons in Bacillus subtilis: evidence for the presence of multiple levels of control of the gapA operon. Mol Microbiol 41, 409-422.PubMed