SbcD

• Description: exonuclease SbcD homolog
  
  

Gene name	sbcD
Synonyms	yixA
Essential	no
Product	exonuclease SbcD homolog
Function	unknown
MW, pI	43 kDa, 5.003
Gene length, protein length	1173 bp, 391 aa
Immediate neighbours	addA, sbcC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

DNA repair/ recombination, genetic competence

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

• Locus tag: BSU10640

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: sbcD family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: Cytoplasm (Homogeneous) PubMed

Database entries

• Structure:

• UniProt: P23479

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: sbcD-sbcC-yisB (according to DBTBS)

• Sigma factor:

• Regulation:
  • positive regulation by ComK PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • GP894 (sbcCD::kan), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537] [WorldCat.org] [DOI] (P p)

Mitsuo Ogura, Hirotake Yamaguchi, Kazuo Kobayashi, Naotake Ogasawara, Yasutaro Fujita, Teruo Tanaka
Whole-genome analysis of genes regulated by the Bacillus subtilis competence transcription factor ComK.
J Bacteriol: 2002, 184(9);2344-51
[PubMed:11948146] [WorldCat.org] [DOI] (P p)

G J Sharples, R G Lloyd
Location of the Bacillus subtilis sbcD gene downstream of addAB, the analogues of E. coli recBC.
Nucleic Acids Res: 1993, 21(8);2010
[PubMed:8493111] [WorldCat.org] [DOI] (P p)