AraL

• Description: sugar phosphate phosphatase, L-arabinose operon
  
  

Gene name	araL
Synonyms	yseA
Essential	no
Product	sugar phosphate phosphatase
Function	detoxification of accidental accumulation of phosphorylated metabolites
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	29 kDa, 5.373
Gene length, protein length	816 bp, 272 aa
Immediate neighbours	araM, araD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

poorly characterized/ putative enzymes

This gene is a member of the following regulons

AraR regulon, CcpA regulon

The gene

Basic information

• Locus tag: BSU28770

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: HAD superfamily

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P94526

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: araA-araB-araD-araL-araM-araN-araP-araQ-abfA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (CcpA) PubMed
  • induced by arabinose (AraR) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • AraR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Isabel Sa-Nogueira, Teresa V Nogueira, Snia Soares, Hermnia de Lencastre
The Bacillus subtilis L-arabinose (ara) operon: nucleotide sequence, genetic organization and expression.
Microbiology (Reading): 1997, 143 ( Pt 3);957-969
[PubMed:9084180] [WorldCat.org] [DOI] (P p)

Lia M Godinho, Isabel de Sá-Nogueira
Characterization and regulation of a bacterial sugar phosphatase of the haloalkanoate dehalogenase superfamily, AraL, from Bacillus subtilis.
FEBS J: 2011, 278(14);2511-24
[PubMed:21575135] [WorldCat.org] [DOI] (I p)

L J Mota, P Tavares, I Sá-Nogueira
Mode of action of AraR, the key regulator of L-arabinose metabolism in Bacillus subtilis.
Mol Microbiol: 1999, 33(3);476-89
[PubMed:10417639] [WorldCat.org] [DOI] (P p)

José Manuel Inácio, Carla Costa, Isabel de Sá-Nogueira
Distinct molecular mechanisms involved in carbon catabolite repression of the arabinose regulon in Bacillus subtilis.
Microbiology (Reading): 2003, 149(Pt 9);2345-2355
[PubMed:12949161] [WorldCat.org] [DOI] (P p)