WalJ

• Description: coordination of cell division with DNA replication
  
  

Gene name	walJ
Synonyms	yycJ
Essential	no
Product	unknown
Function	coordination of cell division with DNA replication
MW, pI	29 kDa, 5.503
Gene length, protein length	804 bp, 268 aa
Immediate neighbours	yyxA, yycI
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

cell division, cell wall synthesis

The gene

Basic information

• Locus tag: BSU40370

Phenotypes of a mutant

• the mutant divides over unsegregated chromosomes more frequently than wild-type cells, and this phenotype is exacerbated when DNA replication is inhibited PubMed
• increased sensitivity to a narrow spectrum of cephalosporin antibiotics PubMed
• disruption of the corresponding gene in B. anthracis results in a mutator phenotype PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: the enzymatic activity of WalJ directly or indirectly affects cell wall metabolism and is required for accurate coordination of cell division with DNA replication PubMed

• Protein family: predicted member of the metallo-β-lactamase superfamily

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: C0SP91

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: walR-walK-yycH-yycI-walJ-yyxA PubMed

• Sigma factor:

• Regulation: expressed during vegetative growth, repressed during stationary phase PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Hanjing Yang, Cameron Sikavi, Katherine Tran, Shauna M McGillivray, Victor Nizet, Madeline Yung, Aileen Chang, Jeffrey H Miller
Papillation in Bacillus anthracis colonies: a tool for finding new mutators.
Mol Microbiol: 2011, 79(5);1276-93
[PubMed:21205011] [WorldCat.org] [DOI] (I p)

C Fabret, J A Hoch
A two-component signal transduction system essential for growth of Bacillus subtilis: implications for anti-infective therapy.
J Bacteriol: 1998, 180(23);6375-83
[PubMed:9829949] [WorldCat.org] [DOI] (P p)

Additional publications: PubMed