DivIB

• Description: cell-division initiation protein (septum formation)
  
  

Gene name	divIB
Synonyms	dds, tms-12
Essential	yes PubMed
Product	cell-division protein
Function	septum formation
Metabolic function and regulation of this protein in SubtiPathways: Cell wall
MW, pI	30 kDa, 9.244
Gene length, protein length	789 bp, 263 aa
Immediate neighbours	murB, ylxW
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

cell division, sporulation proteins, cell envelope stress proteins (controlled by SigM, W, X, Y), essential genes, membrane proteins

This gene is a member of the following regulons

SigE regulon, SigM regulon, SpoIIID regulon

The gene

Basic information

• Locus tag: BSU15240

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ftsQ family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • PbpB-DivIB-(FtsL-DivIC)2 PubMed, as part of the divisome
  • WalK-DivIB PubMed

• Localization: cell membrane PubMed

Database entries

• Structure: 2ALJ (major extracytoplasmic domain, Geobacillus stearothermophilus)

• UniProt: P16655

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• • murE-mraY-murD-spoVE-murG-murB-divIB-ylxW-ylxX-sbp PubMed
  • spoVE-murG-murB-divIB-ylxW-ylxX-sbp PubMed
  • murG-murB-divIB-ylxW-ylxX-sbp PubMed
  • divIB-ylxW-ylxX-sbp PubMed

• Sigma factor:
  • spoVE: SigE PubMed
  • murG: SigMPubMed
  • divIB: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Tatsuya Fukushima, Isako Furihata, Robyn Emmins, Richard A Daniel, James A Hoch, Hendrik Szurmant
A role for the essential YycG sensor histidine kinase in sensing cell division.
Mol Microbiol: 2011, 79(2);503-22
[PubMed:21219466] [WorldCat.org] [DOI] (I p)

Susan L Rowland, Kimberly D Wadsworth, Scott A Robson, Carine Robichon, Jon Beckwith, Glenn F King
Evidence from artificial septal targeting and site-directed mutagenesis that residues in the extracytoplasmic β domain of DivIB mediate its interaction with the divisomal transpeptidase PBP 2B.
J Bacteriol: 2010, 192(23);6116-25
[PubMed:20870765] [WorldCat.org] [DOI] (I p)

Pamela Gamba, Jan-Willem Veening, Nigel J Saunders, Leendert W Hamoen, Richard A Daniel
Two-step assembly dynamics of the Bacillus subtilis divisome.
J Bacteriol: 2009, 191(13);4186-94
[PubMed:19429628] [WorldCat.org] [DOI] (I p)

Warawan Eiamphungporn, John D Helmann
The Bacillus subtilis sigma(M) regulon and its contribution to cell envelope stress responses.
Mol Microbiol: 2008, 67(4);830-48
[PubMed:18179421] [WorldCat.org] [DOI] (P p)

V L Katis, R G Wake, E J Harry
Septal localization of the membrane-bound division proteins of Bacillus subtilis DivIB and DivIC is codependent only at high temperatures and requires FtsZ.
J Bacteriol: 2000, 182(12);3607-11
[PubMed:10852898] [WorldCat.org] [DOI] (P p)

E J Harry, S L Rowland, M S Malo, R G Wake
Expression of divIB of Bacillus subtilis during vegetative growth.
J Bacteriol: 1994, 176(4);1172-9
[PubMed:8106328] [WorldCat.org] [DOI] (P p)

G Theeragool, A Miyao, K Yamada, T Sato, Y Kobayashi
In vivo expression of the Bacillus subtilis spoVE gene.
J Bacteriol: 1993, 175(13);4071-80
[PubMed:8320223] [WorldCat.org] [DOI] (P p)

E J Harry, B J Stewart, R G Wake
Characterization of mutations in divIB of Bacillus subtilis and cellular localization of the DivIB protein.
Mol Microbiol: 1993, 7(4);611-21
[PubMed:8459777] [WorldCat.org] [DOI] (P p)

A O Henriques, H de Lencastre, P J Piggot
A Bacillus subtilis morphogene cluster that includes spoVE is homologous to the mra region of Escherichia coli.
Biochimie: 1992, 74(7-8);735-48
[PubMed:1391053] [WorldCat.org] [DOI] (P p)