CcpN
- Description: transcriptional repressor of gluconeogenetic genes and of sr1. repression in the presence of glucose
Gene name | ccpN |
Synonyms | yqzB |
Essential | no |
Product | transcriptional regulator |
Function | repressor of genes involved in gluconeogenesis (gapB, pckA) and of sr1 |
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate, Cys, Met & Sulfate assimilation, Central C-metabolism | |
MW, pI | 23.4 kDa, 7.22 |
Gene length, protein length | 636 bp, 212 amino acids |
Immediate neighbours | yqfL, glyS |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
Categories containing this gene/protein
carbon core metabolism, regulators of core metabolism
This gene is a member of the following regulons
The CcpN regulon:
The gene
Basic information
- Locus tag: BSU25250
Phenotypes of a mutant
Impaired growth on glucose due to re-routing of carbon from glycolysis to the pentose phosphate pathway PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: transcription repression of the gapB, pckA, and sr1 genes in the presence of glucose PubMed
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- Structure:
- UniProt: O34994
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation: constitutively expressed PubMed
- Additional information:
- the intracellular concentration of CcpN is about 4 myM (according to PubMed).
Biological materials
- Mutant: DB104 ccpN::cat, available in Sabine Brantl lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- Antibody: available in Sabine Brantl lab
Labs working on this gene/protein
Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France
Sabine Brantl, Bacterial Genetics, Friedrich-Schiller-University of Jena, Germany homepage
Uwe Sauer, ETH Zürich, Switzerland homepage
Your additional remarks
References
Reviews
Original Publications