YfiT

• Description: probable metal-dependent hydrolase
  
  

Gene name	yfiT
Synonyms
Essential	no
Product	unknown
Function	unknown
MW, pI	20 kDa, 6.612
Gene length, protein length	534 bp, 178 aa
Immediate neighbours	yfiS, yfiU
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

poorly characterized/ putative enzymes

The gene

Basic information

• Locus tag: BSU08390

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: metal hydrolase yfiT family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): Ni(2+) PubMed

• Effectors of protein activity:

• Interactions: homodimer PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1RXQ

• UniProt: O31562

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yfiT (according to DBTBS)

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Shyamala S Rajan, Xiaojing Yang, Ludmilla Shuvalova, Frank Collart, Wayne F Anderson
YfiT from Bacillus subtilis is a probable metal-dependent hydrolase with an unusual four-helix bundle topology.
Biochemistry: 2004, 43(49);15472-9
[PubMed:15581359] [WorldCat.org] [DOI] (P p)