AdaB

• Description: O6-methylguanine-DNA methyltransferase
  
  

Gene name	adaB
Synonyms
Essential	no
Product	O6-methylguanine-DNA methyltransferase
Function	adaptive response to alkylative DNA damage
MW, pI	19 kDa, 6.152
Gene length, protein length	537 bp, 179 aa
Immediate neighbours	adaA, ndhF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

DNA repair/ recombination

This gene is a member of the following regulons

AdaA regulon

The gene

Basic information

• Locus tag: BSU01820

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine (according to Swiss-Prot)

• Protein family: MGMT family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P19220

• KEGG entry: [3]

• E.C. number: 2.1.1.63

Expression and regulation

• Operon: adaA-adaB PubMed

• Sigma factor: SigA PubMed

• Regulation: positive control by AdaA PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

F Morohoshi, K Hayashi, N Munakata
Bacillus subtilis ada operon encodes two DNA alkyltransferases.
Nucleic Acids Res: 1990, 18(18);5473-80
[PubMed:2120677] [WorldCat.org] [DOI] (P p)