YyaF

• Description: GTP-binding protein/ GTPase
  
  

Gene name	yyaF
Synonyms
Essential	no
Product	GTP-binding protein/ GTPase
Function	unknown
MW, pI	39 kDa, 4.659
Gene length, protein length	1098 bp, 366 aa
Immediate neighbours	rpsF, yyaE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU40920

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

GTP-binding proteins, proteins of unknown function

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP

• Protein family: EngD subfamily (according to Swiss-Prot) Era/Obg family

• Paralogous protein(s): Era, Obg, YphC, YsxC, RbgA, YqeH, ThdF, YyaF, YnbA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P37518

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yyaF-rpsF-ssbA-rpsR PubMed

• Sigma factor:

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed
  • ComK: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:
  • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP836, available in Stülke lab
  • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP844, available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Naotake Ogasawara, Nara, Japan