TcyB

• Description: cystine ABC transporter (permease), membrane protein
  
  

Gene name	tcyB
Synonyms	yckJ
Essential	no
Product	cystine ABC transporter (permease), membrane protein
Function	cystine uptake
MW, pI	26 kDa, 9.932
Gene length, protein length	702 bp, 234 aa
Immediate neighbours	tcyC, tcyA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU03600

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

Categories containing this gene/protein

ABC transporters, biosynthesis/ acquisition of amino acids, membrane proteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ABC transmembrane type-1 domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: TcyC-TcyB-TcyA PubMed

• Localization: membrane protein PubMed

Database entries

• Structure:

• UniProt: P42200

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: tcyA-tcyB-tcyC (putative) PubMed

• Sigma factor:

• Regulation: very weakly expressed under all conditions PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Pierre Burguière, Sandrine Auger, Marie-Françoise Hullo, Antoine Danchin, Isabelle Martin-Verstraete
Three different systems participate in L-cystine uptake in Bacillus subtilis.
J Bacteriol: 2004, 186(15);4875-84
[PubMed:15262924] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)

F Rodriguez, G Grandi
An operon encoding a novel ABC-type transport system in Bacillus subtilis.
Microbiology (Reading): 1995, 141 ( Pt 7);1781-4
[PubMed:7551042] [WorldCat.org] [DOI] (P p)