RecF

• Description: repair, recombination
  
  

Gene name	recF
Synonyms
Essential
Product	unknown
Function	repair, recombination
MW, pI	42 kDa, 7.015
Gene length, protein length	1110 bp, 370 aa
Immediate neighbours	yaaA, yaaB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU00040

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

DNA repair/ recombination

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: recF family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P05651

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yaaA-recF-yaaB-gyrB PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Dawit Kidane, Humberto Sanchez, Juan C Alonso, Peter L Graumann
Visualization of DNA double-strand break repair in live bacteria reveals dynamic recruitment of Bacillus subtilis RecF, RecO and RecN proteins to distinct sites on the nucleoids.
Mol Microbiol: 2004, 52(6);1627-39
[PubMed:15186413] [WorldCat.org] [DOI] (P p)

S Ayora, J C Alonso
Purification and characterization of the RecF protein from Bacillus subtilis 168.
Nucleic Acids Res: 1997, 25(14);2766-72
[PubMed:9207023] [WorldCat.org] [DOI] (P p)

N Ogasawara, S Moriya, H Yoshikawa
Structure and function of the region of the replication origin of the Bacillus subtilis chromosome. IV. Transcription of the oriC region and expression of DNA gyrase genes and other open reading frames.
Nucleic Acids Res: 1985, 13(7);2267-79
[PubMed:2987848] [WorldCat.org] [DOI] (P p)