QueF

• Description: nitrile reductase, synthesis of the modified ribonucleotide queuosine
  
  

Gene name	queF
Synonyms	ykvM
Essential	no
Product	nitrile reductase
Function	tRNA modification
MW, pI	19 kDa, 4.927
Gene length, protein length	495 bp, 165 aa
Immediate neighbours	queE, ykvN
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU13750

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

Categories containing this gene/protein

translation

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 7-aminomethyl-7-carbaguanine + 2 NADP⁺ = 7-cyano-7-carbaguanine + 2 NADPH (according to Swiss-Prot)

• Protein family: QueF type 1 subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O31678

• KEGG entry: [2]

• E.C. number: 1.7.1.13

Expression and regulation

• Operon: queC-queD-queE-queF PubMed

• Sigma factor:

• Regulation:
  • repressed in the presence of queuosine (preQ1 riboswitch) PubMed

• Regulatory mechanism:
  • preQ1 riboswitch: transcriptional antitermination in the absence of queuosine PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: