PucE

• Description: xanthine dehydrogenase
  
  

Gene name	pucE
Synonyms	yurB
Essential	no
Product	xanthine dehydrogenase
Function	purine utilization
Metabolic function and regulation of this protein in SubtiPathways: Purine catabolism, Nucleotides (regulation)
MW, pI	18 kDa, 6.127
Gene length, protein length	519 bp, 173 aa
Immediate neighbours	pucM, pucD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU32470

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

utilization of nucleotides

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Xanthine + NAD⁺ + H₂O = urate + NADH (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1JRO (from Rhodobacter capsulatus, 43% identity, 58% similarity) PubMed

• UniProt: O32143

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: pucA-pucB-pucC-pucD-pucE PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by purine nucleotides (effector: allantoin) (PucR) PubMed

• Regulatory mechanism:
  • PucR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)

Lars Beier, Per Nygaard, Hanne Jarmer, Hans H Saxild
Transcription analysis of the Bacillus subtilis PucR regulon and identification of a cis-acting sequence required for PucR-regulated expression of genes involved in purine catabolism.
J Bacteriol: 2002, 184(12);3232-41
[PubMed:12029039] [WorldCat.org] [DOI] (P p)

A C Schultz, P Nygaard, H H Saxild
Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator.
J Bacteriol: 2001, 183(11);3293-302
[PubMed:11344136] [WorldCat.org] [DOI] (P p)