CysS

• Description: cysteine-tRNA synthetase
  
  

Gene name	cysS
Synonyms	snpA
Essential	yes PubMed
Product	cysteine-tRNA synthetase
Function	translation
Metabolic function and regulation of this protein in SubtiPathways: tRNA charging, Cys, Met & Sulfate assimilation
MW, pI	53 kDa, 5.07
Gene length, protein length	1398 bp, 466 aa
Immediate neighbours	cysE, mrnC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU00940

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

translation, essential genes

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys) (according to Swiss-Prot)

• Protein family: class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Ser-270 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1U0B (from Escherichia coli, 42% identity, 56% similarity) PubMed

• UniProt: Q06752

• KEGG entry: [3]

• E.C. number: 6.1.1.16

Expression and regulation

• Operon: gltX-cysE-cysS-mrnC-yacO-yacP PubMed

• Sigma factor: SigA PubMed

• Regulation: repression by cysteine (T-box) PubMed

• Regulatory mechanism: T-box, overlaps a transcription terminator upstream of cysE: transcription antitermination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)

Y Gagnon, R Breton, H Putzer, M Pelchat, M Grunberg-Manago, J Lapointe
Clustering and co-transcription of the Bacillus subtilis genes encoding the aminoacyl-tRNA synthetases specific for glutamate and for cysteine and the first enzyme for cysteine biosynthesis.
J Biol Chem: 1994, 269(10);7473-82
[PubMed:7510287] [WorldCat.org] (P p)