ArsC

• Description: arsenate reductase
  
  

Gene name	arsC
Synonyms	yqcM
Essential	no
Product	arsenate reductase
Function	detoxification of arsenate
MW, pI	15 kDa, 4.749
Gene length, protein length	417 bp, 139 aa
Immediate neighbours	spoIVCA, arsB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU25780

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

resistance against toxic metals, Skin element

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein tyrosine phosphate + H₂O = protein tyrosine + phosphate (according to Swiss-Prot)

• Protein family: ArsC family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: ArsC-TrxA PubMed

• Localization:

Database entries

• Structure: 1Z2D (reduced form), 1Z2E (oxidized form), 2IPA (TrxA-ArsC-complex) PubMed, 1JL3

• UniProt: P45947

• KEGG entry: [3]

• E.C. number: 3.1.3.48

Expression and regulation

• Operon: arsR-yqcK-arsB-arsC PubMed

• Sigma factor: SigA PubMed, SigF PubMed

• Regulation:
  • expressed early during sporulation in the forespore (SigF) PubMed
  • expressed in the presence of toxic metal ions (As(III), Cd(II) and Ag(I)) (ArsR) PubMed

• Regulatory mechanism:
  • ArsR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

You Li, Yunfei Hu, Xinxin Zhang, Huimin Xu, Ewen Lescop, Bin Xia, Changwen Jin
Conformational fluctuations coupled to the thiol-disulfide transfer between thioredoxin and arsenate reductase in Bacillus subtilis.
J Biol Chem: 2007, 282(15);11078-83
[PubMed:17303556] [WorldCat.org] [DOI] (P p)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Z Guan, L Hederstedt, J Li, X D Su
Preparation and crystallization of a Bacillus subtilis arsenate reductase.
Acta Crystallogr D Biol Crystallogr: 2001, 57(Pt 11);1718-21
[PubMed:11679756] [WorldCat.org] [DOI] (P p)

T Sato, Y Kobayashi
The ars operon in the skin element of Bacillus subtilis confers resistance to arsenate and arsenite.
J Bacteriol: 1998, 180(7);1655-61
[PubMed:9537360] [WorldCat.org] [DOI] (P p)