LexA

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  • Description: transcriptional repressor of the SOS regulon <br/><br/>
Gene name lexA
Synonyms dinR
Essential no
Product transcriptional repressor of the SOS regulon
Function regulation of DNA damage repair
Function and regulation of this protein in SubtiPathways: <br/>Phosphorelay
MW, pI 22 kDa, 5.155
Gene length, protein length 615 bp, 205 aa
Immediate neighbours fosB, yneA
Get the DNA and protein sequences <br/> (Barbe et al., 2009)
Genetic context <br/> LexA context.gif
<div align="right"> <small>This image was kindly provided by SubtiList</small></div>

<br/><br/><br/><br/><br/><br/>

The gene

Basic information

  • Locus tag: BSU17850

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Represses uvrB, dinB, dinC, recA genes and itself by binding to the 14 bp palindromic sequence 5'-CGAACNNNNGTTCG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. (according to Swiss-Prot)
  • Protein family: peptidase S24 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure: 1JHH (from E. coli, S119A mutant, 33% identity, 52% similarity) PubMed
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism: autorepression
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

<pubmed>8899710, 9045831 9555905 8969214, 1657879,,16267290 11532142 11551506 16549676 </pubmed>