GltA

---

---

Under Construction! Please Visit Reserve Page. Page Will Be Available Shortly

---

CLICK HERE

---

• Description: large subunit of glutamate synthase <br/><br/>

Gene name	gltA
Synonyms
Essential	no
Product	glutamate synthase (large subunit)
Function	glutamate biosynthesis
Metabolic function and regulation of this protein in SubtiPathways: <br/>Ammonium/ glutamate
MW, pI	168 kDa, 5.47
Gene length, protein length	4560 bp, 1520 amino acids
Immediate neighbours	gltB, gltC
Get the DNA and protein sequences <br/> (Barbe et al., 2009)
Genetic context <br/> <div align="right"> <small>This image was kindly provided by SubtiList</small></div>

<br/><br/><br/><br/><br/><br/>

The gene

Basic information

• Locus tag: BSU18450

Phenotypes of a mutant

auxotrophic for glutamate

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2 L-glutamate + NADP⁺ = L-glutamine + 2-oxoglutarate + NADPH (according to Swiss-Prot) 2 L-glutamate + NADP(+) <=> L-glutamine + 2-oxoglutarate + NADPH

• Protein family: glutamate synthase family (according to Swiss-Prot) glutamate synthase family

• Paralogous protein(s): YerD

Extended information on the protein

• Kinetic information:

• Domains:
  • Glutamine amidotransferase type-2 domain (22-415)
  • Nucleotide binding domain (1060-1112)

• Modification:

• Cofactor(s): 3Fe-4S, FAD, FMN

• Effectors of protein activity:

• Interactions:
  • GltA-GltB PubMed
  • GltA-Icd PubMed

• Localization: membrane associated PubMed, cytoplasm

Database entries

• Structure:

• UniProt: P39812

• KEGG entry: [3]

• E.C. number: 1.4.1.13 3 1.4.1.13]

Additional information

subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

• Operon: gltA-gltB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expression activated by glucose (11 fold) (CcpA, GltC) PubMed
  • repressed by arginine (GltC, RocG) PubMed
  • expressed in the presence of ammonium PubMed
  • repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed

• Regulatory mechanism:
  • GltC: transcription activation PubMed
  • TnrA: transcription repression PubMed

• Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

• Mutant: GP807 (del gltAB::tet), GP222 (gltA under the control of p-xyl), available in Stülke lab

• Expression vector:

• lacZ fusion: pGP526 (in pAC7), pGP919 (in pAC5), available in Stülke lab

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody:

Labs working on this gene/protein

Linc Sonenshein, Tufts University, Boston, MA, USA Homepage

Jörg Stülke, University of Göttingen, Germany Homepage

References

<pubmed>12823818,,11029411,12850135 7559360 15150225 2548995 17183217, 17608797,17134717,14523131,12823818, 18326565,17981983,18763711 20933603 17012385 </pubmed>