Sandbox

Steps on the way to an artificial organism

Daniel G Gibson, John I Glass, Carole Lartigue, Vladimir N Noskov, Ray-Yuan Chuang, Mikkel A Algire, Gwynedd A Benders, Michael G Montague, Li Ma, Monzia M Moodie, Chuck Merryman, Sanjay Vashee, Radha Krishnakumar, Nacyra Assad-Garcia, Cynthia Andrews-Pfannkoch, Evgeniya A Denisova, Lei Young, Zhi-Qing Qi, Thomas H Segall-Shapiro, Christopher H Calvey, Prashanth P Parmar, Clyde A Hutchison, Hamilton O Smith, J Craig Venter
Creation of a bacterial cell controlled by a chemically synthesized genome.
Science: 2010, 329(5987);52-6
[PubMed:20488990] [WorldCat.org] [DOI] (I p)

Carole Lartigue, Sanjay Vashee, Mikkel A Algire, Ray-Yuan Chuang, Gwynedd A Benders, Li Ma, Vladimir N Noskov, Evgeniya A Denisova, Daniel G Gibson, Nacyra Assad-Garcia, Nina Alperovich, David W Thomas, Chuck Merryman, Clyde A Hutchison, Hamilton O Smith, J Craig Venter, John I Glass
Creating bacterial strains from genomes that have been cloned and engineered in yeast.
Science: 2009, 325(5948);1693-6
[PubMed:19696314] [WorldCat.org] [DOI] (I p)

Daniel G Gibson, Gwynedd A Benders, Cynthia Andrews-Pfannkoch, Evgeniya A Denisova, Holly Baden-Tillson, Jayshree Zaveri, Timothy B Stockwell, Anushka Brownley, David W Thomas, Mikkel A Algire, Chuck Merryman, Lei Young, Vladimir N Noskov, John I Glass, J Craig Venter, Clyde A Hutchison, Hamilton O Smith
Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome.
Science: 2008, 319(5867);1215-20
[PubMed:18218864] [WorldCat.org] [DOI] (I p)

Carole Lartigue, John I Glass, Nina Alperovich, Rembert Pieper, Prashanth P Parmar, Clyde A Hutchison, Hamilton O Smith, J Craig Venter
Genome transplantation in bacteria: changing one species to another.
Science: 2007, 317(5838);632-8
[PubMed:17600181] [WorldCat.org] [DOI] (I p)

Metabolism

Frederik M Meyer, Jan Gerwig, Elke Hammer, Christina Herzberg, Fabian M Commichau, Uwe Völker, Jörg Stülke
Physical interactions between tricarboxylic acid cycle enzymes in Bacillus subtilis: evidence for a metabolon.
Metab Eng: 2011, 13(1);18-27
[PubMed:20933603] [WorldCat.org] [DOI] (I p)

Ying Zhang, Ines Thiele, Dana Weekes, Zhanwen Li, Lukasz Jaroszewski, Krzysztof Ginalski, Ashley M Deacon, John Wooley, Scott A Lesley, Ian A Wilson, Bernhard Palsson, Andrei Osterman, Adam Godzik
Three-dimensional structural view of the central metabolic network of Thermotoga maritima.
Science: 2009, 325(5947);1544-9
[PubMed:19762644] [WorldCat.org] [DOI] (I p)

Bryson D Bennett, Elizabeth H Kimball, Melissa Gao, Robin Osterhout, Stephen J Van Dien, Joshua D Rabinowitz
Absolute metabolite concentrations and implied enzyme active site occupancy in Escherichia coli.
Nat Chem Biol: 2009, 5(8);593-9
[PubMed:19561621] [WorldCat.org] [DOI] (I p)

Wataru Iwasaki, Toshihisa Takagi
Rapid pathway evolution facilitated by horizontal gene transfers across prokaryotic lineages.
PLoS Genet: 2009, 5(3);e1000402
[PubMed:19266023] [WorldCat.org] [DOI] (I p)

Fabian M Commichau, Fabian M Rothe, Christina Herzberg, Eva Wagner, Daniel Hellwig, Martin Lehnik-Habrink, Elke Hammer, Uwe Völker, Jörg Stülke
Novel activities of glycolytic enzymes in Bacillus subtilis: interactions with essential proteins involved in mRNA processing.
Mol Cell Proteomics: 2009, 8(6);1350-60
[PubMed:19193632] [WorldCat.org] [DOI] (I p)

Armen Y Mulkidjanian, Eugene V Koonin, Kira S Makarova, Sergey L Mekhedov, Alexander Sorokin, Yuri I Wolf, Alexis Dufresne, Frédéric Partensky, Henry Burd, Denis Kaznadzey, Robert Haselkorn, Michael Y Galperin
The cyanobacterial genome core and the origin of photosynthesis.
Proc Natl Acad Sci U S A: 2006, 103(35);13126-31
[PubMed:16924101] [WorldCat.org] [DOI] (P p)

M Ribbe, D Gadkari, O Meyer
N2 fixation by Streptomyces thermoautotrophicus involves a molybdenum-dinitrogenase and a manganese-superoxide oxidoreductase that couple N2 reduction to the oxidation of superoxide produced from O2 by a molybdenum-CO dehydrogenase.
J Biol Chem: 1997, 272(42);26627-33
[PubMed:9334244] [WorldCat.org] [DOI] (P p)