PGP1389
Revision as of 06:40, 18 October 2010 by MLehnik (talk | contribs) (Created page with "File:PGP1389 Subtiwiki.png '''General comments''' Integrative plasmid for the fusion of Streptag tag to the C-terminus of a protein, keeping the control of the expression u...")
General comments
Integrative plasmid for the fusion of Streptag tag to the C-terminus of a protein, keeping the control of the expression under its natural promotor
- E.coli: multicopy plasmid, select for ampicillin
- B. subtilis: no ori, select for spectinomycin
Hints for the usage
- amplify the last 600bp of your gene of interest,
- skip the Stopp-Codon of the gene in the reverse primer
- take care that the integration is in-frame
