MenD

• Description: 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase / 2-oxoglutarate decarboxylase
  
  

Gene name	menD
Synonyms
Essential	yes PubMed
Product	2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase / 2-oxoglutarate decarboxylase
Function	biosynthesis of menaquinone
Metabolic function and regulation of this protein in SubtiPathways: Menaquinone
MW, pI	63 kDa, 5.655
Gene length, protein length	1740 bp, 580 aa
Immediate neighbours	ytxM, menF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU30820

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO₂ (according to Swiss-Prot)

• Protein family: MenD subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): thiamine pyrophosphate, divalent metal ion such as Mn(2+)) PubMed

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3LQ1 (from Listeria monocytogenes)

• UniProt: P23970

• KEGG entry: [3]

• E.C. number: 2.2.1.9

Expression and regulation

• Operon: menF-menD-ytxM-menB-menE-menC PubMed

• Sigma factor: SigA: PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Alice Dawson, Minjiao Chen, Paul K Fyfe, Zhihong Guo, William N Hunter
Structure and reactivity of Bacillus subtilis MenD catalyzing the first committed step in menaquinone biosynthesis.
J Mol Biol: 2010, 401(2);253-64
[PubMed:20600129] [WorldCat.org] [DOI] (I p)

Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221] [WorldCat.org] [DOI] (P p)

X Qin, H W Taber
Transcriptional regulation of the Bacillus subtilis menp1 promoter.
J Bacteriol: 1996, 178(3);705-13
[PubMed:8550504] [WorldCat.org] [DOI] (P p)

J R Driscoll, H W Taber
Sequence organization and regulation of the Bacillus subtilis menBE operon.
J Bacteriol: 1992, 174(15);5063-71
[PubMed:1629163] [WorldCat.org] [DOI] (P p)