CsrA

• Description: similar to E. coli carbon storage regulator
  
  

Gene name	csrA
Synonyms	yviG
Essential	no
Product	unknown
Function	unknown
MW, pI	8 kDa, 6.09
Gene length, protein length	222 bp, 74 aa
Immediate neighbours	hag, fliW
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU35370

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: csrA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: CsrA-RsmY (hypothetical)

• Localization:

Database entries

• Structure: 1T3O

• UniProt: P33911

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: csrA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP469 (spc), available in Stülke lab

• Expression vector:
  • pGP381 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
  • for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP383, available in Stülke lab

• lacZ fusion: pGP461 (in pAC7), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Original Publications

Helen Yakhnin, Pallavi Pandit, Tom J Petty, Carol S Baker, Tony Romeo, Paul Babitzke
CsrA of Bacillus subtilis regulates translation initiation of the gene encoding the flagellin protein (hag) by blocking ribosome binding.
Mol Microbiol: 2007, 64(6);1605-20
[PubMed:17555441] [WorldCat.org] [DOI] (P p)

Prajna R Kulkarni, Xiaohui Cui, Joshua W Williams, Ann M Stevens, Rahul V Kulkarni
Prediction of CsrA-regulating small RNAs in bacteria and their experimental verification in Vibrio fischeri.
Nucleic Acids Res: 2006, 34(11);3361-9
[PubMed:16822857] [WorldCat.org] [DOI] (I e)

B Soldo, V Lazarevic, C Mauël, D Karamata
Sequence of the 305 degrees-307 degrees region of the Bacillus subtilis chromosome.
Microbiology (Reading): 1996, 142 ( Pt 11);3079-88
[PubMed:8969505] [WorldCat.org] [DOI] (P p)