PGP1087

pGP1087: click to enlarge

General comments

Integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping the control of the expression under its natural promotor

• E.coli: lowcopy plasmid, select for ampicillin
• B. subtilis: no ori, select for erythromycin/lincomycin

Hints for the usage

• amplify the last 300bp of your gene of interest,
• skip the Stop-Codon of the gene in the revers primer
• take care that the integration is in-frame

Antibody

• dilute the anitbody 1:10000 (you can freeze the Blotto solution and reuse it)