PGP1331

General comments

Integrative Plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping the control of the expression under its natural promotor

• E.coli: multicopy plasmid, select for ampicillin
• B. subtilis: no ori, select for spectinomycin

Hints for the usage amplify the last 600bp of your gene of interest, skip the Stopp-Codon of the gene in the revers primer take care that the integration is in-frame

Antibody dilute the anitbody 1:10000 (you can freeze the Blotto solution and reuse it)