PucM

• Description: uricase
  
  

Gene name	pucM
Synonyms	yunM
Essential	no
Product	uricase
Function	purine utilization
Metabolic function and regulation of this protein in SubtiPathways: Purine catabolism, Nucleotides (regulation)
MW, pI	13 kDa, 5.082
Gene length, protein length	363 bp, 121 aa
Immediate neighbours	pucL, pucE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU32460

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 5-hydroxyisourate + H₂O = 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate (according to Swiss-Prot)

• Protein family: 5-hydroxyisourate hydrolase subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2H0E

• UniProt: O32142

• KEGG entry: [3]

• E.C. number: 3.5.2.17

Expression and regulation

• Operon:
  • pucR-pucJ-pucK-pucL-pucM PubMed
  • pucJ-pucK-pucL-pucM PubMed

• Sigma factor:
  • pucJ: SigA PubMed

• Regulation:
  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed

• Regulatory mechanism:
  • TnrA: transcription activation PubMed
  • PucR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Pollyana Pfrimer, Lidia Maria Pepe de Moraes, Alexsandro Sobreira Galdino, Loise Pedrosa Salles, Viviane Castelo Branco Reis, Janice Lisboa De Marco, Maura Vianna Prates, Carlos Bloch, Fernando Araripe Gonçalves Torres
Cloning, purification, and partial characterization of Bacillus subtilis urate oxidase expressed in Escherichia coli.
J Biomed Biotechnol: 2010, 2010;674908
[PubMed:20168977] [WorldCat.org] [DOI] (I p)

Du-Kyo Jung, Youra Lee, Sung Goo Park, Byoung Chul Park, Ghyung-Hwa Kim, Sangkee Rhee
Structural and functional analysis of PucM, a hydrolase in the ureide pathway and a member of the transthyretin-related protein family.
Proc Natl Acad Sci U S A: 2006, 103(26);9790-5
[PubMed:16782815] [WorldCat.org] [DOI] (P p)

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)

Lars Beier, Per Nygaard, Hanne Jarmer, Hans H Saxild
Transcription analysis of the Bacillus subtilis PucR regulon and identification of a cis-acting sequence required for PucR-regulated expression of genes involved in purine catabolism.
J Bacteriol: 2002, 184(12);3232-41
[PubMed:12029039] [WorldCat.org] [DOI] (P p)

A C Schultz, P Nygaard, H H Saxild
Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator.
J Bacteriol: 2001, 183(11);3293-302
[PubMed:11344136] [WorldCat.org] [DOI] (P p)