HisA

• Description: phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase, homologue to HisF
  
  

Gene name	hisA
Synonyms
Essential	no
Product	phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase, homologue to HisF
Function	biosynthesis of histidine
Metabolic function and regulation of this protein in SubtiPathways: His
MW, pI	26 kDa, 4.842
Gene length, protein length	735 bp, 245 aa
Immediate neighbours	hisF, hisH
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU34880

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxyribulos-1-ylamino)methylideneamino)-1-(5-phosphoribosyl)imidazole-4-carboxamide (according to Swiss-Prot)

• Protein family: hisA/hisF family (according to Swiss-Prot)

• Paralogous protein(s): HisF

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 2VEP (from Streptomyces coelicolor, 41% identity, 58% similarity) PubMed

• UniProt: O35006

• KEGG entry: [2]

• E.C. number: 5.3.1.16

Expression and regulation

• Operon: hisZ-hisG-hisD-hisB-hisH-hisA-hisF-hisI PubMed

• Sigma factor:

• Regulation: repressed by casamino acids PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)