SirA

• Description: sporulation protein, inhibits DNA replication
  
  

Gene name	sirA
Synonyms	yneE, yoxF
Essential	no
Product	inhibitor of DNA replication
Function	control of chromosome copy number
MW, pI	17 kDa, 7.353
Gene length, protein length	444 bp, 148 aa
Immediate neighbours	tkt, yneF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

yneE was renamed to sirA.

Basic information

• Locus tag: BSU17900

Phenotypes of a mutant

"over-replication" during sporulation PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: interacts with DnaA and displaces it from the replication origin, resulting in inhibition of replication PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: SirA-DnaA PubMed

• Localization:

Database entries

• Structure:

• UniProt: P45707

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: sirA-yneF PubMed

• Sigma factor:

• Regulation:
  • expressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Your additional remarks

sirA (sporulation inhibitor of replication) is activated by Spo0A~P and inhibits DNA replication during sporulation.

References

Reviews

Tsutomu Katayama, Shogo Ozaki, Kenji Keyamura, Kazuyuki Fujimitsu
Regulation of the replication cycle: conserved and diverse regulatory systems for DnaA and oriC.
Nat Rev Microbiol: 2010, 8(3);163-70
[PubMed:20157337] [WorldCat.org] [DOI] (I p)