PhrE

• Description: response regulator aspartate phosphatase (RapE) regulator, control of the phosphorelay
  
  

Gene name	phrE
Synonyms
Essential	no
Product	phosphatase (RapE) regulator
Function	control of sporulation initiation
Function and regulation of this protein in SubtiPathways: Phosphorelay
MW, pI	4 kDa, 8.863
Gene length, protein length	132 bp, 44 aa
Immediate neighbours	rapE, yqzI
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU25840

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: phr family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O32025

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • rapE-phrE PubMed
  • phrE PubMed

• Sigma factor:
  • phrE: SigA PubMed, SigH PubMed

• Regulation: repressed by glucose (10-fold) (CcpA) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism: CcpA: transcription repression
  • CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)

Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455] [WorldCat.org] [DOI] (P p)

R S McQuade, N Comella, A D Grossman
Control of a family of phosphatase regulatory genes (phr) by the alternate sigma factor sigma-H of Bacillus subtilis.
J Bacteriol: 2001, 183(16);4905-9
[PubMed:11466295] [WorldCat.org] [DOI] (P p)

M Jiang, R Grau, M Perego
Differential processing of propeptide inhibitors of Rap phosphatases in Bacillus subtilis.
J Bacteriol: 2000, 182(2);303-10
[PubMed:10629174] [WorldCat.org] [DOI] (P p)