YloS

• Description: thiamine pyrophosphokinase
  
  

Gene name	yloS
Synonyms	thiN
Essential	no
Product	thiamine pyrophosphokinase
Function	thiamine salvage
Metabolic function and regulation of this protein in SubtiPathways: Thiamin
MW, pI	23 kDa, 5.216
Gene length, protein length	642 bp, 214 aa
Immediate neighbours	rpe, spoVM
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU15800

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + thiamine = AMP + thiamine diphosphate (according to Swiss-Prot)

• Protein family: thiamine pyrophosphokinase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3CQ9 (from Lactobacillus plantarum, 38% identity, 55% similarity)

• UniProt: O34664

• KEGG entry: [2]

• E.C. number: 2.7.6.2

Expression and regulation

• Operon:

• Sigma factor: SigE PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Christopher T Jurgenson, Tadhg P Begley, Steven E Ealick
The structural and biochemical foundations of thiamin biosynthesis.
Annu Rev Biochem: 2009, 78;569-603
[PubMed:19348578] [WorldCat.org] [DOI] (I p)

Original publications

Ghislain Schyns, Sébastien Potot, Yi Geng, Teresa M Barbosa, Adriano Henriques, John B Perkins
Isolation and characterization of new thiamine-deregulated mutants of Bacillus subtilis.
J Bacteriol: 2005, 187(23);8127-36
[PubMed:16291685] [WorldCat.org] [DOI] (P p)

Jonathan Melnick, Ewa Lis, Joo-Heon Park, Cynthia Kinsland, Hirotada Mori, Tomoya Baba, John Perkins, Ghislain Schyns, Olga Vassieva, Andrei Osterman, Tadhg P Begley
Identification of the two missing bacterial genes involved in thiamine salvage: thiamine pyrophosphokinase and thiamine kinase.
J Bacteriol: 2004, 186(11);3660-2
[PubMed:15150256] [WorldCat.org] [DOI] (P p)