GidA

• Description: glucose-inhibited division protein, tRNA uridine 5-carboxymethylaminomethyl modification enzyme
  
  

Gene name	gidA
Synonyms
Essential	no
Product	tRNA uridine 5-carboxymethylaminomethyl modification enzyme
Function	tRNA modification
MW, pI	69 kDa, 6.326
Gene length, protein length	1884 bp, 628 aa
Immediate neighbours	rsmG, thdF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU41010

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: mnmG family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: GidA-ThdF (suggested by interaction of E. coli counterparts PubMed and by co-localization on the chromosome)

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 3G05 (the protein of E. coli, 51% identity)

• UniProt: P25812

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: thdF-gidA-rsmG-noc PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:
  • ComK: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Rong Shi, Magda Villarroya, Rafael Ruiz-Partida, Yunge Li, Ariane Proteau, Silvia Prado, Ismaïl Moukadiri, Alfonso Benítez-Páez, Rodrigo Lomas, John Wagner, Allan Matte, Adrián Velázquez-Campoy, M-Eugenia Armengod, Miroslaw Cygler
Structure-function analysis of Escherichia coli MnmG (GidA), a highly conserved tRNA-modifying enzyme.
J Bacteriol: 2009, 191(24);7614-9
[PubMed:19801413] [WorldCat.org] [DOI] (I p)

Mitsuo Ogura, Hirotake Yamaguchi, Kazuo Kobayashi, Naotake Ogasawara, Yasutaro Fujita, Teruo Tanaka
Whole-genome analysis of genes regulated by the Bacillus subtilis competence transcription factor ComK.
J Bacteriol: 2002, 184(9);2344-51
[PubMed:11948146] [WorldCat.org] [DOI] (P p)

Jörg Sievers, Brian Raether, Marta Perego, Jeff Errington
Characterization of the parB-like yyaA gene of Bacillus subtilis.
J Bacteriol: 2002, 184(4);1102-11
[PubMed:11807071] [WorldCat.org] [DOI] (P p)