OpuBD

• Description: choline ABC transporter (membane protein)
  
  

Gene name	opuBD
Synonyms	proZ
Essential	no
Product	choline ABC transporter (membane protein)
Function	compatible solute transport
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	23 kDa, 10.06
Gene length, protein length	678 bp, 226 aa
Immediate neighbours	yvaQ, opuBC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU33700

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: CysTW subfamily (according to Swiss-Prot)

• Paralogous protein(s): OpuCD

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P39775

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: opuBA-opuBB-opuBC-opuBD PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by salt stress PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

R M Kappes, B Kempf, S Kneip, J Boch, J Gade, J Meier-Wagner, E Bremer
Two evolutionarily closely related ABC transporters mediate the uptake of choline for synthesis of the osmoprotectant glycine betaine in Bacillus subtilis.
Mol Microbiol: 1999, 32(1);203-16
[PubMed:10216873] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)

G Nau-Wagner, J Boch, Le Good JA, E Bremer
High-affinity transport of choline-O-sulfate and its use as a compatible solute in Bacillus subtilis.
Appl Environ Microbiol: 1999, 65(2);560-8
[PubMed:9925583] [WorldCat.org] [DOI] (P p)