GanB

• Description: arabinogalactan endo-1,4-beta-galactosidase
  
  

Gene name	yvfO
Synonyms
Essential	no
Product	arabinogalactan endo-1,4-beta-galactosidase
Function	galactan utilization
MW, pI	47 kDa, 8.243
Gene length, protein length	1287 bp, 429 aa
Immediate neighbours	rsbP, lacA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU34120

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Endohydrolysis of (1->4)-beta-D-galactosidic linkages in arabinogalactans (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 53 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2J74 (B. licheniformis)

• UniProt: O07013

• KEGG entry: [3]

• E.C. number: 3.2.1.89

Expression and regulation

• Operon: cycB-yvfL-yvfM-lacA-yvfO PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:
  • LacR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Stephanie Shipkowski, Jean E Brenchley
Bioinformatic, genetic, and biochemical evidence that some glycoside hydrolase family 42 beta-galactosidases are arabinogalactan type I oligomer hydrolases.
Appl Environ Microbiol: 2006, 72(12);7730-8
[PubMed:17056685] [WorldCat.org] [DOI] (P p)

R A Daniel, J Haiech, F Denizot, J Errington
Isolation and characterization of the lacA gene encoding beta-galactosidase in Bacillus subtilis and a regulator gene, lacR.
J Bacteriol: 1997, 179(17);5636-8
[PubMed:9287030] [WorldCat.org] [DOI] (P p)