AhrC

• Description: AhrC represses the genes for arginine biosynthesis and activates the genes for arginine catabolism.
  
  

Gene name	ahrC
Synonyms	argR
Essential	no
Product	transcriptional regulator
Function	transcriptional regulator of arginine metabolic genes
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate
MW, pI	16,7 kDa, 5.52
Gene length, protein length	447 bp, 149 amino acids
Immediate neighbours	yqxC, recN
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU24250

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: transcriptional activator/ repressor of genes involved in arginine metabolism

• Protein family: argR family (according to Swiss-Prot)

• Paralogous protein(s):

Genes controlled by AhrC

• Activation by AhrC: rocA-rocB-rocC, rocD-rocE-rocF, rocG

• Repression by AhrC: argC-argJ-argB-argD-carA-carB-argF, argG-argH

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): L-arginine is the co-factor required for transcription repression/ activation

• Effectors of protein activity:

• Interactions:

• Localization: Cytoplasm

Database entries

• Structure: 2P5M (C-Terminus), 2P5K (complex with an 18bp DNA operator), 2P5L (N-terminus), 1F9N, NCBI PubMed,N-Terminus NCBI, C-Terminus NCBI, complex with an 18bp DNA operator NCBI

• UniProt: P17893

• KEGG entry: [3]

Expression and regulation

• Operon: ahrC-recN PubMed

• Sigma factor:

• Regulation:
  • by sRNA sr1

• Regulatory mechanism:
  • inhibtion of translation upon binding of sr1 to the ahrC mRNA PubMed

• Additional information:

Biological materials

• Mutant: GP729 (aphA3), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• Antibody:

Labs working on this gene/protein

Simon Phillips, Leeds University, UK Homepage

Michel Debarbouille, Pasteur Institute, Paris, France Homepage