DfrA

• Description: dihydrofolate reductase
  
  

Gene name	dfrA
Synonyms	tmp
Essential	yes PubMed
Product	dihydrofolate reductase
Function	biosynthesis of folate
Metabolic function and regulation of this protein in SubtiPathways: Nucleotides (regulation), Folate
MW, pI	19 kDa, 5.219
Gene length, protein length	504 bp, 168 aa
Immediate neighbours	ypkP, thyB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU21810

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 5,6,7,8-tetrahydrofolate + NADP⁺ = 7,8-dihydrofolate + NADPH (according to Swiss-Prot)

• Protein family: spmB family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: DfrA-ThyB

• Localization:

Database entries

• Structure: 1ZDR (Geobacillus stearothermophilus)

• UniProt: P11045

• KEGG entry: [2]

• E.C. number: 1.5.1.3

Expression and regulation

• Operon: thyB-dfrA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

M Iwakura, M Kawata, K Tsuda, T Tanaka
Nucleotide sequence of the thymidylate synthase B and dihydrofolate reductase genes contained in one Bacillus subtilis operon.
Gene: 1988, 64(1);9-20
[PubMed:2840350] [WorldCat.org] [DOI] (P p)

T T Myoda, V L Funanage
Coregulation of dihydrofolate reductase and thymidylate synthase B in bacillus subtilis.
Biochim Biophys Acta: 1985, 824(2);99-103
[PubMed:3918568] [WorldCat.org] [DOI] (P p)

T T Myoda, S V Lowther, V L Funanage, F E Young
Cloning and mapping of the dihydrofolate reductase gene of Bacillus subtilis.
Gene: 1984, 29(1-2);135-43
[PubMed:6436144] [WorldCat.org] [DOI] (P p)