BglH

• Description: phospho-beta-glucosidase
  
  

Gene name	bglH
Synonyms
Essential	no
Product	phospho-beta-glucosidase
Function	salicin utilization
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	53 kDa, 4.957
Gene length, protein length	1407 bp, 469 aa
Immediate neighbours	yxiE, bglP
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU39260

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H₂O = D-glucose + D-glucose 6-phosphate (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 1 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P40740

• KEGG entry: [3]

• E.C. number: 3.2.1.86

Expression and regulation

• Operon: bglP-bglH-yxiE PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (CcpA, LicT) PubMed
  • induced by salicin (LicT) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression, (CcpA binding site overlaps -35 region) PubMed
  • LicT-dependent RNA switch (antitermination, lack of LicT-dependent antitermination in the presence of gucose due to the requirement of LicT to be phosphorylated by HPr PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: