LexA
- Description: transcriptional repressor of the SOS regulon
Gene name | lexA |
Synonyms | dinR |
Essential | no |
Product | transcriptional repressor of the SOS regulon |
Function | regulation of DNA damage repair |
MW, pI | 22 kDa, 5.155 |
Gene length, protein length | 615 bp, 205 aa |
Immediate neighbours | fosB, yneA |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Locus tag: BSU17850
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Represses dinA, dinB, dinC, recA genes and itself by binding to the 14 bp palindromic sequence 5'-CGAACNNNNGTTCG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. (according to Swiss-Prot)
- Protein family: peptidase S24 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: P31080
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: lexA
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
K W Winterling, D Chafin, J J Hayes, J Sun, A S Levine, R E Yasbin, R Woodgate
The Bacillus subtilis DinR binding site: redefinition of the consensus sequence.
J Bacteriol: 1998, 180(8);2201-11
[PubMed:9555905]
[WorldCat.org]
[DOI]
(P p)
K W Winterling, A S Levine, R E Yasbin, R Woodgate
Characterization of DinR, the Bacillus subtilis SOS repressor.
J Bacteriol: 1997, 179(5);1698-703
[PubMed:9045831]
[WorldCat.org]
[DOI]
(P p)
M C Miller, J B Resnick, B T Smith, C M Lovett
The bacillus subtilis dinR gene codes for the analogue of Escherichia coli LexA. Purification and characterization of the DinR protein.
J Biol Chem: 1996, 271(52);33502-8
[PubMed:8969214]
[WorldCat.org]
(P p)
B J Haijema, D van Sinderen, K Winterling, J Kooistra, G Venema, L W Hamoen
Regulated expression of the dinR and recA genes during competence development and SOS induction in Bacillus subtilis.
Mol Microbiol: 1996, 22(1);75-85
[PubMed:8899710]
[WorldCat.org]
[DOI]
(P p)