SpoIVA

• Description: required for proper spore cortex formation and coat assembly
  
  

Gene name	spoIVA
Synonyms	spoVP
Essential	no
Product	basement layer protein for spore coat assembly
Function	spore cortex formation and coat assembly
MW, pI	55 kDa, 4.546
Gene length, protein length	1476 bp, 492 aa
Immediate neighbours	hbs, yphF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU22800

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P35149

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor: SigE PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Kumaran S Ramamurthi, Richard Losick
ATP-driven self-assembly of a morphogenetic protein in Bacillus subtilis.
Mol Cell: 2008, 31(3);406-14
[PubMed:18691972] [WorldCat.org] [DOI] (I p)

Kumaran S Ramamurthi, Katie Rose Clapham, Richard Losick
Peptide anchoring spore coat assembly to the outer forespore membrane in Bacillus subtilis.
Mol Microbiol: 2006, 62(6);1547-57
[PubMed:17427285] [WorldCat.org] [DOI] (P p)

Katerina Ragkousi, Patrick Eichenberger, Christiaan van Ooij, Peter Setlow
Identification of a new gene essential for germination of Bacillus subtilis spores with Ca2+-dipicolinate.
J Bacteriol: 2003, 185(7);2315-29
[PubMed:12644503] [WorldCat.org] [DOI] (P p)

K D Price, R Losick
A four-dimensional view of assembly of a morphogenetic protein during sporulation in Bacillus subtilis.
J Bacteriol: 1999, 181(3);781-90
[PubMed:9922240] [WorldCat.org] [DOI] (P p)

Peter J Lewis, Jeffery Errington
Use of green fluorescent protein for detection of cell-specific gene expression and subcellular protein localization during sporulation in Bacillus subtilis.
Microbiology (Reading): 1996, 142 ( Pt 4);733-740
[PubMed:8936302] [WorldCat.org] [DOI] (P p)

K Pogliano, E Harry, R Losick
Visualization of the subcellular location of sporulation proteins in Bacillus subtilis using immunofluorescence microscopy.
Mol Microbiol: 1995, 18(3);459-70
[PubMed:8748030] [WorldCat.org] [DOI] (P p)

C D Webb, A Decatur, A Teleman, R Losick
Use of green fluorescent protein for visualization of cell-specific gene expression and subcellular protein localization during sporulation in Bacillus subtilis.
J Bacteriol: 1995, 177(20);5906-11
[PubMed:7592342] [WorldCat.org] [DOI] (P p)

A Driks, S Roels, B Beall, C P Moran, R Losick
Subcellular localization of proteins involved in the assembly of the spore coat of Bacillus subtilis.
Genes Dev: 1994, 8(2);234-44
[PubMed:8299942] [WorldCat.org] [DOI] (P p)

C M Stevens, R Daniel, N Illing, J Errington
Characterization of a sporulation gene, spoIVA, involved in spore coat morphogenesis in Bacillus subtilis.
J Bacteriol: 1992, 174(2);586-94
[PubMed:1729247] [WorldCat.org] [DOI] (P p)

S Roels, A Driks, R Losick
Characterization of spoIVA, a sporulation gene involved in coat morphogenesis in Bacillus subtilis.
J Bacteriol: 1992, 174(2);575-85
[PubMed:1729246] [WorldCat.org] [DOI] (P p)

L B Zheng, R Losick
Cascade regulation of spore coat gene expression in Bacillus subtilis.
J Mol Biol: 1990, 212(4);645-60
[PubMed:1691789] [WorldCat.org] [DOI] (P p)