YqiQ

• Description: methylisocitrate lyase
  
  

Gene name	yqiQ
Synonyms	prpB
Essential	no
Product	methylisocitrate lyase
Function	mother cell metabolism
Metabolic function and regulation of this protein in SubtiPathways: Coenzyme A, Central C-metabolism
MW, pI	32 kDa, 5.279
Gene length, protein length	903 bp, 301 aa
Immediate neighbours	yqzF, mmgE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU24120

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate (according to Swiss-Prot)

• Protein family: Methylisocitrate lyase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure: 1MUM (enzyme of E. coli)

• UniProt: P54528

• KEGG entry: [3]

• E.C. number: 4.1.3.30

Expression and regulation

• Operon:

• Sigma factor: SigE PubMed

• Regulation: repressed by glucose (CcpA)

• Regulatory mechanism: CcpA: transcription repression

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Clemens Grimm, Andreas Evers, Matthias Brock, Claudia Maerker, Gerhard Klebe, Wolfgang Buckel, Klaus Reuter
Crystal structure of 2-methylisocitrate lyase (PrpB) from Escherichia coli and modelling of its ligand bound active centre.
J Mol Biol: 2003, 328(3);609-21
[PubMed:12706720] [WorldCat.org] [DOI] (P p)