Sda
- Description: Sda is a checkpoint protein that is upregulated in response to replication stress (Burkholder et al 2001, Cell). Sda inhibits the autokinase activity of KinA (and likely KinB as well). This in its turn results in reduced levels of Spo0A~P. Thus, replication stressed cells will not initiate sporulation
Sda is rapidly turned over by the ClpP/X system because of some uncharged residues at its C-terminus Ruvolo et al 2006, MolMic.
| Gene name | sda |
| Synonyms | |
| Essential | no |
| Product | developmental checkpoint protein |
| Function | mediates a developmental checkpoint coupling initiation of
sporulation (phosphorylation of Spo0A) to the function of replication initiation proteins |
| MW, pI | 6 kDa, 5.605 |
| Gene length, protein length | 156 bp, 52 aa |
| Immediate neighbours | yloV, sdaAA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU25690
Phenotypes of a mutant
- sporulates at a higher frequency
- will sporulate in the presence of replication stress
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- UniProt: Q7WY62
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: sda
- Sigma factor: SigA
- Regulatory mechanism: Probably activated by DnaA under replication stress
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Bill Burkholder http://burkholder.stanford.edu/
Your additional remarks
References
