PdxK

• Description: pyridoxine, pyridoxal, and pyridoxamine kinase
  
  

Gene name	pdxK
Synonyms	ywdB, ipa-52r, thiD
Essential	no
Product	pyridoxine, pyridoxal, and pyridoxamine kinase
Function	biosynthesis of pyridoxal phosphate
MW, pI	28 kDa, 4.922
Gene length, protein length	813 bp, 271 aa
Immediate neighbours	ywdC, ywdA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU38020

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + 4-amino-2-methyl-5-phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine (according to Swiss-Prot)

• Protein family: thiD family (according to Swiss-Prot)

• Paralogous protein(s): ThiD

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2IB5

• UniProt: P39610

• KEGG entry: [3]

• E.C. number: 2.7.4.7

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Joseph A Newman, Sanjan K Das, Svetlana E Sedelnikova, David W Rice
Cloning, purification and preliminary crystallographic analysis of a putative pyridoxal kinase from Bacillus subtilis.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2006, 62(Pt 10);1006-9
[PubMed:17012797] [WorldCat.org] [DOI] (I p)

Joo-Heon Park, Kristin Burns, Cynthia Kinsland, Tadhg P Begley
Characterization of two kinases involved in thiamine pyrophosphate and pyridoxal phosphate biosynthesis in Bacillus subtilis: 4-amino-5-hydroxymethyl-2methylpyrimidine kinase and pyridoxal kinase.
J Bacteriol: 2004, 186(5);1571-3
[PubMed:14973012] [WorldCat.org] [DOI] (P p)