ParB

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  • Description: chromosome positioning near the pole and transport through the polar septum / antagonist of Soj-dependent inhibition of sporulation initiation, traps DNA loops, required for the recruitment of the SMC complex to the origin of replication

Gene name spo0J
Synonyms parB, spo0JB
Essential no
Product site-specific DNA-binding protein
Function chromosome positioning before asymmetric septation,

specifying its orientation, and imposing directionality on

its subsequent transport through the septum; required for sporulation

(antagonist of Soj-dependent inhibition of sporulation initiation)

Gene expression levels in SubtiExpress: spo0J
Interactions involving this protein in SubtInteract: Spo0J
MW, pI 32 kDa, 8.417
Gene length, protein length 846 bp, 282 aa
Immediate neighbours yyaC, soj
Sequences Protein DNA DNA_with_flanks
Genetic context
Spo0J context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Spo0J expression.png















Categories containing this gene/protein

DNA condensation/ segregation, sporulation/ other

This gene is a member of the following regulons

Spo0A regulon

The gene

Basic information

  • Locus tag: BSU40960

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • inhibits Soj dimerization and concomitant DNA-binding activity by stimulating Soj ATPase activity PubMed
    • recruits the condensin complex to the DNA origin region PubMed
    • the dimer binds specifcally to the centromere-like parS sequence PubMed
  • Protein family: parB family (according to Swiss-Prot)
  • Paralogous protein(s): Noc

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 341 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 362 PubMed
    • number of protein molecules per cell: 880 (equivalent to 440 dimers) PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Rodrigo Reyes-Lamothe, Emilien Nicolas, David J Sherratt
Chromosome replication and segregation in bacteria.
Annu Rev Genet: 2012, 46;121-43
[PubMed:22934648] [WorldCat.org] [DOI] (I p)

Original publications