CdaR

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  • Description: effector protein controlling CdaA diadenylate cyclase activity

Gene name cdaR
Synonyms ybbR
Essential no
Product effector protein controlling CdaA diadenylate cyclase activity
Function regulation of c-di-AMP synthesis
Gene expression levels in SubtiExpress: cdaR
Interactions involving this protein in SubtInteract: CdaR
Metabolic function and regulation of this protein in SubtiPathways:
cdaR
MW, pI 52 kDa, 4.719
Gene length, protein length 1449 bp, 483 aa
Immediate neighbours cdaA, glmM
Sequences Protein DNA DNA_with_flanks
Genetic context
YbbR context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YbbR expression.png















Categories containing this gene/protein

metabolism of signalling nucleotides

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU01760

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • stimulates CdaA activity upon protein-protein interaction PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information: the cdaA-cdaR part of the mRNA is very stable (half-life > 15 min) PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 60 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 138 PubMed

Biological materials

  • Expression vector:
    • IPTG inducible expression of Strep-cdaR in E. coli: pGP2565 (in pGP172), available in Jörg Stülke's lab
    • IPTG inducible expression of His-cdaR in E. coli: pGP2557 (in pET19b), available in Jörg Stülke's lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab. Respective plasmids: pGP1989 and pGP1992.

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original publications

Felix M P Mehne, Katrin Gunka, Hinnerk Eilers, Christina Herzberg, Volkhard Kaever, Jörg Stülke
Cyclic di-AMP homeostasis in bacillus subtilis: both lack and high level accumulation of the nucleotide are detrimental for cell growth.
J Biol Chem: 2013, 288(3);2004-17
[PubMed:23192352] [WorldCat.org] [DOI] (I p)

Yun Luo, John D Helmann
Analysis of the role of Bacillus subtilis σ(M) in β-lactam resistance reveals an essential role for c-di-AMP in peptidoglycan homeostasis.
Mol Microbiol: 2012, 83(3);623-39
[PubMed:22211522] [WorldCat.org] [DOI] (I p)

Adam W Barb, John R Cort, Jayaraman Seetharaman, Scott Lew, Hsiau-Wei Lee, Thomas Acton, Rong Xiao, Michael A Kennedy, Liang Tong, Gaetano T Montelione, James H Prestegard
Structures of domains I and IV from YbbR are representative of a widely distributed protein family.
Protein Sci: 2011, 20(2);396-405
[PubMed:21154411] [WorldCat.org] [DOI] (I p)

Jun Yin, Paul D Straight, Shaun M McLoughlin, Zhe Zhou, Alison J Lin, David E Golan, Neil L Kelleher, Roberto Kolter, Christopher T Walsh
Genetically encoded short peptide tag for versatile protein labeling by Sfp phosphopantetheinyl transferase.
Proc Natl Acad Sci U S A: 2005, 102(44);15815-20
[PubMed:16236721] [WorldCat.org] [DOI] (P p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)