Nos

• Description: nitric-oxide synthase
  
  

Gene name	nos
Synonyms	yflM
Essential	no
Product	nitric-oxide synthase
Function	production of nitric oxide
Gene expression levels in SubtiExpress: nos
Interactions involving this protein in SubtInteract: Nos
MW, pI	38 kDa, 5.528
Gene length, protein length	1008 bp, 336 aa
Immediate neighbours	yflN, yflL
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

miscellaneous metabolic pathways, biosynthesis of antibacterial compounds

The gene

Basic information

• Locus tag: BSU07630

Database entries

• BsubCyc: BSU07630

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: Bacterial NOS oxygenase subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): heme PubMed

• Effectors of protein activity:

• Interactions:
  • Nos-YkuN (electron transfer from to Nos) PubMed

• Localization:

Database entries

• BsubCyc: BSU07630

• Structure: 2AMO (loose dimer), 1M7V (bound to tetrahydrofolate and arginine)

• UniProt: O34453

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: nos-yflK (according to DBTBS)

• Expression browser: nos PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information: Note that the gene yflL is located between nos and yflK, but on the opposite strand.
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 18 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: