YvcL

• Description: WhiA family protein, involved in Z ring assembly, putative transcription factor
  
  

Gene name	yvcL
Synonyms
Essential	no
Product	cell division protein
Function	control of Z ring asembly
Gene expression levels in SubtiExpress: yvcL
MW, pI	36 kDa, 9.479
Gene length, protein length	948 bp, 316 aa
Immediate neighbours	crh, yvcK
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell division, transcription factors and their control

The gene

Basic information

• Locus tag: BSU34750

Phenotypes of a mutant

• a yvcL zapA double mutant grows poorly, and the cells are filamentous (due to a defect in Z ring assembly) PubMed
• a yvcL ezrA double mutant grows poorly, and the cells are filamentous PubMed
• a yvcL minC-minD mutant grows poorly, and the cells are filamentous PubMed
• a yvcL noc double mutant grows poorly, and the cells are filamentous PubMed
• the defects of the yvcL zapA double mutant can be suppressed by inactivation of either gtaB, pgcA, or ugtP PubMed

Database entries

• BsubCyc: BSU34750

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: WhiA family PubMed

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: suggested HTH domain at the N terminus PubMed

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:
  • localizes to the nucleoid PubMed

Database entries

• BsubCyc: BSU34750

• Structure:
  • 3HYI (Crystal structure of full-length DUF199/WhiA from Thermotoga maritima)
  • 3HYJ (Crystal structure of the N-terminal LAGLIDADG domain of DUF199/WhiA)

• UniProt: O06975

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yvcI-yvcJ-yvcK-yvcL-crh-yvcN PubMed

• Expression browser: yvcL PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • constitutive expression at both protein and RNA levels PubMed
  • very weak stimuation of expression by citrate and succinate PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP231 (amyE::PrpsJ-lacZ, yvcL::cat) available in Jörg Stülke's lab; plasmid pGP1468 was used to disrupt the gene.

• Expression vector:
  • pGP1469 for expression in B. subtilis (in pGP382, with C-terminal STREP-TAG), available in Jörg Stülke's lab
  • pGP2429 (N-terminal Strep-tag, purification from E. coli and/or B. subtilis, in pDG148), for SPINE, available in Jörg Stülke's lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's and Boris Görke's labs

• Antibody: available in Leendert Hamoen's lab PubMed

Your additional remarks

WhiA orthologues are present in all Gram-positive bacteria and best characterized by the WhiA sporulation control factor in Streptomyces coelicolor. They are thought to act as genetic regulators that have evolved from LAGLIDADG homing endonucleases (LHE).