SsrA

• Description: tmRNA
  
  

Gene name	ssrA
Synonyms
Essential	no
Product	tmRNA
Function	trans-translation
Gene length	bp
Immediate neighbours	yvaG, smpB
Gene sequence (+200bp)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

translation, ncRNA

The gene

Basic information

• Locus tag:

• Interactions: SmpB-SsrA

Phenotypes of a mutant

• impaired growth at high and low temperatures PubMed
• inactivation of ssrA restores beta-lactam resistance in a sigM mutant PubMed

Database entries

• BsubCyc: BSU_misc_RNA_55

• DBTBS entry: no entry

• SubtiList entry: [1]

• Structure:

Expression and regulation

• Operon:
  • secG-yvaK-rnr-smpB-ssrA PubMed
  • yvaK-rnr-smpB-ssrA PubMed
  • smpB-ssrA PubMed
  • ssrA PubMed

• Sigma factor:
  • yvaK: SigB PubMed

• Regulation:
  • yvaK: induced by stress (SigB) PubMed

• Additional information:

Biological materials

• Mutant: GP323 (cat), available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/RNA

• Chet Price, Davis, USA homepage

References

Reviews

Noël Molière, Kürşad Turgay
General and regulatory proteolysis in Bacillus subtilis.
Subcell Biochem: 2013, 66;73-103
[PubMed:23479438] [WorldCat.org] [DOI] (P p)

Janine Kirstein, Noël Molière, David A Dougan, Kürşad Turgay
Adapting the machine: adaptor proteins for Hsp100/Clp and AAA+ proteases.
Nat Rev Microbiol: 2009, 7(8);589-99
[PubMed:19609260] [WorldCat.org] [DOI] (I p)

Jeffrey H Withey, David I Friedman
A salvage pathway for protein structures: tmRNA and trans-translation.
Annu Rev Microbiol: 2003, 57;101-23
[PubMed:12730326] [WorldCat.org] [DOI] (P p)