RuvA

• Description: Holliday junction DNA helicase, part of the RuvA-RuvB branch migration translocase
  
  

Gene name	ruvA
Synonyms
Essential	no
Product	Holliday junction DNA helicase
Function	DNA repair/ recombination
Gene expression levels in SubtiExpress: ruvA
Interactions involving this protein in SubtInteract: RuvA
MW, pI	22 kDa, 5.384
Gene length, protein length	603 bp, 201 aa
Immediate neighbours	ruvB, bofC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination

This gene is a member of the following regulons

LexA regulon

The gene

Basic information

• Locus tag: BSU27740

Phenotypes of a mutant

• the inactivations of recU and ruvA-ruvB are synthetically lethal PubMed

Database entries

• BsubCyc: BSU27740

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ruvA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • RuvA-RuvB

• Localization:

Database entries

• BsubCyc: BSU27740

• Structure:

• UniProt: O05392

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ruvA-ruvB PubMed

• Expression browser: ruvA PubMed

• Sigma factor:

• Regulation:
  • induced upon DNA damage (LexA) PubMed

• Regulatory mechanism:
  • LexA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A896 (no resistance), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Original publications

Cristina Cañas, Yuki Suzuki, Chiara Marchisone, Begoña Carrasco, Verónica Freire-Benéitez, Kunio Takeyasu, Juan C Alonso, Silvia Ayora
Interaction of branch migration translocases with the Holliday junction-resolving enzyme and their implications in Holliday junction resolution.
J Biol Chem: 2014, 289(25);17634-46
[PubMed:24770420] [WorldCat.org] [DOI] (I p)

Nora Au, Elke Kuester-Schoeck, Veena Mandava, Laura E Bothwell, Susan P Canny, Karen Chachu, Sierra A Colavito, Shakierah N Fuller, Eli S Groban, Laura A Hensley, Theresa C O'Brien, Amish Shah, Jessica T Tierney, Louise L Tomm, Thomas M O'Gara, Alexi I Goranov, Alan D Grossman, Charles M Lovett
Genetic composition of the Bacillus subtilis SOS system.
J Bacteriol: 2005, 187(22);7655-66
[PubMed:16267290] [WorldCat.org] [DOI] (P p)