BshA

• Description: L-malic acid glycosyltransferase, involved in bacillithiol synthesis
  
  

Gene name	bshA
Synonyms	jojH, ypjH
Essential	no
Product	L-malic acid glycosyltransferase
Function	biosynthesis of bacillithiol
Gene expression levels in SubtiExpress: bshA
MW, pI	41 kDa, 6.149
Gene length, protein length	1131 bp, 377 aa
Immediate neighbours	cca, bshB1
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

miscellaneous metabolic pathways, resistance against oxidative and electrophile stress

This gene is a member of the following regulons

Spx regulon

The gene

Basic information

• Locus tag: BSU22460

Database entries

• BsubCyc: BSU22460

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: UDP-GlcNAc + L-malate = GlcNAc(α1→2)L-malate PubMed; also uses D-malate as a substrate, but with much lower affinity PubMed

• Protein family: NamA subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • subject to feedback inhibition by bacillithiol PubMed

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU22460

• Structure: 3MBO (from B. anthracis) PubMed

• UniProt: P42982

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: ypjD-dapB-mgsA-bshB1-bshA-cca-birA PubMed

• Expression browser: bshA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by diamide stess (thiol depletion) (Spx) PubMed

• Regulatory mechanism:
  • Spx: transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 168 PubMed

Biological materials

• Mutant:
  • bshA::mls available in John_Helmann lab
  • GP88 (bshA::pX2(cat)), available in Jörg Stülke's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: