Rnc

• Description: RNase III; required for the degradation of sense/antisense transcripts in Gram positive, low GC bacteria
  
  

Gene name	rnc
Synonyms	rncS
Essential	yes
Product	endoribonuclease III
Function	cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (scr)
Gene expression levels in SubtiExpress: rnc
Metabolic function and regulation of this protein in SubtiPathways: Rnc
MW, pI	28 kDa, 8.076
Gene length, protein length	747 bp, 249 aa
Immediate neighbours	acpA, smc
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

Rnases, translation, protein secretion, essential genes

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU15930

Phenotypes of a mutant

• essential PubMed
• the rnc gene can be deleted in strains cured of the Skin element and SPß prophage (or upon deletion of the toxin genes txpA and yonT) PubMed

Database entries

• BsubCyc: BSU15930

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • Endonucleolytic cleavage of double-stranded RNA to 5'-phosphomonoester (according to Swiss-Prot)
  • cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (scr)
  • degradation of txpA mRNA-RatA RNA duplex (encoded on the Skin element) PubMed
  • degradation of yonT mRNA-as-yonT RNA duplex (encoded on the SP-beta prophage) PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU15930

• Structure:

• UniProt: P51833

• KEGG entry: [3]

• E.C. number: 3.1.26.3

Expression and regulation

• Operon: rnc-smc-ftsY PubMed

• Expression browser: rnc PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody:

Labs working on this gene/protein

David Bechhofer, Mount Sinai School, New York, USA Homepage

Ciaran Condon, IBPC, Paris, France Homepage

References

Original publications

Durand S, Gilet L, Condon C  
The essential function of B. subtilis RNase III is to silence foreign toxin genes. 
PLoS Genet. 2012 8(12): e1003181.
PubMed:23300471